FCMPASS Overview
Current Feature Support
If your cytometer does not appear in the table below please get in contact with us and provide an example of a .fcs file from your cytometer at fcmpass@nih.gov.
Cytometer | Fluorescent Calibration |
Light Scatter Calibration |
Detector Optimisation |
Automated Cross-Calibration |
---|---|---|---|---|
Amnis Imagestream | ||||
Amnis CellStream | ||||
Apogee A50 | ||||
BD FACS Aria I/II/III | ||||
BD FACS Canto | ||||
BD LSR Fortessa | ||||
BD Influx | ||||
BD Symphony A3/A5/S6 | ||||
BC Astrios | ||||
BC CytoFLEX (S/LX) | ||||
Cytek Aurora | ||||
Cytek Northern Lights | ||||
NovoCyte Quanteon | ||||
TF Attune NxT | ||||
nanoFCM |
MIFlowCyt Completion
Framework Criteria | Autocomplete |
---|---|
1.1. Purpose | |
1.2. Keywords | |
1.3. Experiment Variables | |
1.4. Organization name and address | |
1.5. Primary contact name and email address | |
1.6. Date or time period of experiment | |
1.7. Conclusions | |
1.8. Quality control measures | |
2.1.1.1. (2.1.2.1., 2.1.3.1.) Sample description | |
2.1.1.2. Biological sample source description | |
2.1.1.3. Biological sample source organism description | |
2.1.2.2. Environmental sample location | |
2.3. Sample treatment description | |
2.4. Fluorescence reagent(s) description | |
3.1. Instrument manufacturer | |
3.2. Instrument model | |
3.3. Instrument configuration and settings | |
4.1. List-mode data files: We recommend all authors to submit their data files to http://flowrepository.org and to make them available for the peer-review process | |
4.2. Compensation description | |
4.3. Data transformation details | |
4.4.1. Gate description | |
4.4.2. Gate statistics | |
4.4.3. Gate boundaries |
MIFlowCyt-EV Completion
Framework Criteria | Autocomplete |
---|---|
1.1 Preanalytical variables conforming to MISEV guidelines | |
1.2 Experimental design according to MIFlowCyt guidelines | |
2.1 Sample staining details | |
2.2 Sample washing details | |
2.3 Sample dilution details | |
3.1 Buffer alone controls | |
3.2 Buffer with reagent controls | |
3.3 Unstained controls | |
3.4 Isotype controls | |
3.5 Single-stained controls | |
3.6 Procedural controls | |
3.7 Serial dilutions | |
3.8. Detergent treated EV-samples | |
4.1 Trigger Channel(s) and Threshold(s) | |
4.2 Flow Rate / Volumetric quantification | |
4.3 Fluorescence Calibration | |
4.4 Light Scatter Calibration | |
5.1 EV diameter/surface area/volume approximation | |
5.2 EV refractive index approximation | |
5.3 EV epitope number approximation | |
6.1 Completion of MIFlowCyt checklist | |
6.2 Calibrated channel detection range | |
6.3 EV number/concentration | |
6.4 EV brightness | |
7.1. Sharing of data to a public repository |