FCMPASS Overview


Current Feature Support

If your cytometer does not appear in the table below please get in contact with us and provide an example of a .fcs file from your cytometer at fcmpass@nih.gov.

Cytometer Fluorescent
Calibration
Light Scatter
Calibration
Detector
Optimisation
Automated
Cross-Calibration
Amnis Imagestream
Amnis CellStream
Apogee A50
BD FACS Aria I/II/III
BD FACS Canto
BD LSR Fortessa
BD Influx
BD Symphony A3/A5/S6
BC Astrios
BC CytoFLEX (S/LX)
Cytek Aurora
Cytek Northern Lights
NovoCyte Quanteon
TF Attune NxT
nanoFCM

MIFlowCyt Completion

Framework Criteria Autocomplete
1.1. Purpose
1.2. Keywords
1.3. Experiment Variables
1.4. Organization name and address
1.5. Primary contact name and email address
1.6. Date or time period of experiment
1.7. Conclusions
1.8. Quality control measures
2.1.1.1. (2.1.2.1., 2.1.3.1.) Sample description
2.1.1.2. Biological sample source description
2.1.1.3. Biological sample source organism description
2.1.2.2. Environmental sample location
2.3. Sample treatment description
2.4. Fluorescence reagent(s) description
3.1. Instrument manufacturer
3.2. Instrument model
3.3. Instrument configuration and settings
4.1. List-mode data files: We recommend all authors to submit their data files to http://flowrepository.org and to make them available for the peer-review process
4.2. Compensation description
4.3. Data transformation details
4.4.1. Gate description
4.4.2. Gate statistics
4.4.3. Gate boundaries

MIFlowCyt-EV Completion

Framework Criteria Autocomplete
1.1 Preanalytical variables conforming to MISEV guidelines
1.2 Experimental design according to MIFlowCyt guidelines
2.1 Sample staining details
2.2 Sample washing details
2.3 Sample dilution details
3.1 Buffer alone controls
3.2 Buffer with reagent controls
3.3 Unstained controls
3.4 Isotype controls
3.5 Single-stained controls
3.6 Procedural controls
3.7 Serial dilutions
3.8. Detergent treated EV-samples
4.1 Trigger Channel(s) and Threshold(s)
4.2 Flow Rate / Volumetric quantification
4.3 Fluorescence Calibration
4.4 Light Scatter Calibration
5.1 EV diameter/surface area/volume approximation
5.2 EV refractive index approximation
5.3 EV epitope number approximation
6.1 Completion of MIFlowCyt checklist
6.2 Calibrated channel detection range
6.3 EV number/concentration
6.4 EV brightness
7.1. Sharing of data to a public repository