FCMPASS Detector Optimisation: Data Generation


An up-to-date protocol for the acquiring of 8-peak detector setting incremenetation data can be found in the protocol below.

Auto-generating Cytek Aurora Gain Templates

  1. To increase the ergonomics of running detector setting incrementation on the Cytek Aurora, FCMPASS can auto-generate fixed detector template settings that will not normalize to QC settings unless the templates are modified and saved.
Experiment calibration
  1. Navigate to the detector optimzation module from the start menu. Click 'Menu' and select 'Voltration Template Generator'.
Experiment calibration
  1. Modify the gains and threshold accordingly (use the protocol above to aid the selection of settings) and click 'Generate Template'.
  2. Select a folder to exported the generated templates to.

The settings listed are a guide and may be sub-optimal for your specific instrument.