FCMPASS Directory is now hidden and not user defined
FCMPASS directory importing now facilitated
Catalogue
Cytometer cataloguing now supported.
Rainbow multipeak particle cataloguing now supported.
Automated bead cross-calibration supported
MIFlowCyt-EV
Experiment Calibration
Surface area to diameter and volume to diameter fluorescence
transformations supported
Weight regression supported by default to increase regression
exptrapolation reliability.
Datasets no longer static--deleting calibration particles in
catalogue
will trigger software to check any datasets using calibration
particles
and will warn the user.
Duplicating experiments now possible.
Support for negative transforms now possible when using logarithmic
regressions.
Cytometer specific bead cross-calibrations in catalogye can be used
for
experimental calibration.
Duplicating experiments now possible.
FCMPASS Output
Cross-calibration reporting
MIFlowCyt report outputted
Instrument settings outputted
Summary page
MIFlowCyt-EV automation improved
.exRNA file written for nanoflow repository integration
Release Notes:
Update: Quanteon fcs files now supported.
Update: MESF calibration accuracy improved.
Update: Failed .fcs writes are now logged in ErrorLog.txt for
debugging.
Release Notes:
Update: FACSAria fcs files now supported.
Update: extracting threshold settings from fcs file
made more reliable across instrument platforms and
acquisition software versions.
Release Notes:
New: Software looks for updates on start-up.
Bug fix: output report no longer results in error when
writing multiple fluorescence calibrations with
varying reference bead array sizes.
Update: removal of redunatant preferences from v2.
Update: database has improved accuracy with scattering
cross section units.
New: bead catalogue can now append reference bead sets
and reference bead information.
Bug fix: bead catalogue no longer has errors when
adding multiple overlapping bead sets.
Release Notes:
Bug fix: updates to handle experiments with multiple
thresholds per channel
Release Notes:
Bug fix: written fcs files now work with FCSExpress
Release Notes:
Update: Calibration figures are exported into .png
files rather than .ps
Release Notes:
Bug fix: unable to remove bead set from catalogue
Release Notes:
Bug fix: unable to add beads to catalogue on some
computers
Release Notes:
New: Software user interface update enabling cytometer
database and dataset tracking
New: Fluorescence and light scatter calibration bead
cataloguing
New: Fluorescence calibration allows for F:P ratio
alterations
New: Light scatter calibration allows for multiple
customisable core-shell and homogeneous sphere
compositions
New: Light scatter calibration autoatically adjusts
for refractive index across models when wavelength is
altered
New: Light scatter calibration is ~10x faster to
calculate with storage being ~5x smaller
New: fcs file outputs now retain cytometer metadata
from the original files
New: Output folder contains spreadsheet with
MIFlowCyt-EV report and figures for all calibrations
reference in the report
Release Notes:
Fixed bug with writing scatter-cross section to .fcs
file
Fixed bug creating multiple timestamp folders if
export took longer than 1 minute
Release Notes:
Fixed bug where multiple scattering cross-section
conversion parameters were listed if calculated
multiple times
New: error message appears if criteria not fulfilled
when conducting fluorescence
New: plot diameter and scattering axis can be resized
without recalculating
Release Notes:
Fixed bug saving scatter bead datasets
Fixed bug saving MESF bead datasets
Fixed bug stopping writing to some .fcs files
Fixed bug with RI conversions
Fixed bug indexing custom angles
Release Notes:
New: Updated user interface and loading screens
New: more efficient database storage and search system
New: Creation of error log file in database folder
New: Light scatter modelling up to 800 nm illumination
wavelengths
Fixed error stopping softwate update search
Fixed error in the database storage v2.12
Increased start-up speed
New: Added user preferences for default input settings
New: Added control over a number of graph properties
New: Ability to turn parallel computing off (issue for
some users)
New: Added further metadata for data tracking and
reporting
New: Added ability to transpose scatter data
New: Added help tab in menu bar for reporting bugs and
linking how-to guides
New: Scatter-Diameter plot does not plot threshold
when set to 0
New: Exported .fcs file data folders are unique
stopping overwriting
Fixed spelling error in scatter diameter plot legend
New: Adds ability to plot datapoints without using the
data to approximate collection angle
New: Adds ability to plot predicted light scatter
curves without needing reference bead data input
New: Adds ability to remove predicted vesicle light
scatter range from scat-diam plot.
New: Adds ability to customize vesicle channel
intercept diameter
New: Adds ability to remove EV curves entirely
Predicted vs. Acquired plot changed to
'Fold-Difference' by default due to better accuracy.
New: Adds coloured reference background to the
'Fold-Difference' plot indicating whether beads fit
well or not.
Fixed bug stopping the deletion of light scatter bead
datasets
Release Notes:
Fixed bug causing crash if directory window was
cancelled upon startup
Fixed bug in setting minimum y-axis limits for
scatter-diameter curve
New: Added predicted vs. acquired scatter ratio plot
New: Added ability to manually update bead directory
Release Notes:
New: No limit to input values (previously capped at
10)
New: Database integration avoids re-calculation of the
same data twice
New: fcs file integration
New: Diameter determination from fcs
New: RI determination from fcs
New: SSC to scatter-cross section conversion
New: Write parameters to .fcs file format
New: Refractive index-diameter plot output
New: Predicted vs. Acquired scatter regression output